The Nadkarni Lab — Research and Publications

Advanced confocal microscopy of inflammatory histopathologic components in strokeAdvanced confocal microscopy of inflammatory histopathologic components in stroke

RESEARCH

Our NIH funded project investigates the interactions of neutrophils and cerebrovascular blood flow in mouse models of stroke. The lab uses transgenic fluorescent mice, optical coherence tomography and targeted immunologic manipulation in a mouse model of large vessel occlusion. We believe that understanding these relationships and spatiotemporal differences can lead to a better understanding in developing precision therapies for the innate immunity in stroke.

 

Optical imaging of mouse vasculature
Optical imaging of mouse vasculature

 

The Center for Healthy Aging at Stony Brook University has recently funded our lab for a translational clinical study investigating the innate immunity (neutrophils, monocytes and microglia) in both elderly and young stroke patients with large vessel occlusion and subsequent recanalization. We hope to obtain pilot data that demonstrates differences between immunologic responses as a function of age.

 

Laser Speckle, MRI and TTC of a stroke Image 1    Laser Speckle, MRI and TTC of a stroke Image 2
Laser Speckle, MRI and  TTC of a stroke

 

Lastly, in concert with Bioinformatics, we are also in the early stages of banking stroke tissue and physiologic data from our mice to understand variations in the model as it relates to animal behavior.

 

Cranial window of a mouse brain
Cranial window of a mouse brain

 

PUBLICATIONS

  1. Li Y, Schappell LE, Polizu C, DiPersio J, Tsirka SE, Halterman MW, Nadkarni NA. Evolving Clinical-Translational Investigations of Cerebroprotection in Ischemic Stroke. J Clin Med. 2023 Oct 24;12(21):6715. doi: 10.3390/jcm12216715. PMID: 37959180; PMCID: PMC10649331. 
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10649331/
     
  2. Nadkarni NA, Arias E, Fang R, Haynes ME, Zhang HF, Muller WA, Batra A, Sullivan DP. Platelet Endothelial Cell Adhesion Molecule (PECAM/CD31) Blockade Modulates Neutrophil Recruitment Patterns and Reduces Infarct Size in Experimental Ischemic Stroke. Am J Pathol. 2022 Nov;192(11):1619-1632. doi: 10.1016/j.ajpath.2022.07.008. Epub 2022 Aug 8. PMID: 35952762; PMCID: PMC9667712. 
    https://www.sciencedirect.com/science/article/pii/S0002944022002127
     
  3. Nadkarni NA, Maas MB, Batra A, Kim M, Manno EM, Sorond FA, Prabhakaran S, Naidech AM, Liotta EM. Elevated Cerebrospinal Fluid Protein Is Associated with Unfavorable Functional Outcome in Spontaneous Subarachnoid Hemorrhage. J Stroke Cerebrovasc Dis. 2020 Apr;29(4):104605. doi: 10.1016/j.jstrokecerebrovasdis.2019.104605. Epub 2020 Jan 11. PMID: 31932209; PMCID: PMC7066873.
    https://pubmed.ncbi.nlm.nih.gov/31932209/
     
  4. Beckmann L, Zhang X, Nadkarni NA, Cai Z, Batra A, Sullivan DP, Muller WA, Sun C, Kuranov R, Zhang HF. Longitudinal deep-brain imaging in mouse using visible-light optical coherence tomography through chronic microprism cranial window. Biomed Opt Express. 2019 Sep 18;10(10):5235-5250. doi: 10.1364/BOE.10.005235. PMID: 31646044; PMCID: PMC6788609.
    https://pubmed.ncbi.nlm.nih.gov/31646044/

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